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HON 499: Spring 2017 Professor Moon's Library Instruction (Dr. Duncan): SH

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e-articles in Science Direct

Exploring the potential of genome editing CRISPR-Cas9 technology

 
  • a Institute of Systems and Synthetic Biology, Genopole, CNRS, UEVE, Université Paris-Saclay, 5 rue Henri Desbruères, 91030 Évry, France
  • b Chemical and Synthetic Biology Group, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India
 

e-articles

Efficient Gene Editing in Primary Human T Cells

Author: Yvonne Y Chen Affiliation: Department of Chemical and Biomolecular Engineering, University of California—Los Angeles, Los Angeles, CA 90095, USA
Edition/Format: Article Article : English
Publication: Trends in Immunology, v36 n11 (201511): 667-669
  Peer-reviewed
Database: Copyright 2017 Elsevier B.V. All rights reserved
Summary:
Recent advances in T-cell therapy for cancer, viral infections, and autoimmune diseases highlight the broad therapeutic potential of T-cell engineering. However, site-specific genetic manipulation in primary human T cells remains challenging. Two recent studies describe efficient genome editing in T cells using CRISPR and TALEN approaches.

e-articles

Self-targeting by CRISPR: gene regulation or autoimmunity?

Author: A Stern Affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.; L Keren; O Wurtzel; G Amitai; R Sorek
Edition/Format: Article Article : English
Publication: Trends in genetics : TIG, 2010 Aug; 26(8): 335-40
  Peer-reviewed
Database: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Other Databases: ArticleFirstWorldCatBritish Library Serials
Summary:
The recently discovered prokaryotic immune system known as CRISPR (clustered regularly interspaced short palindromic repeats) is based on small RNAs ('spacers') that restrict phage and plasmid infection. It has been hypothesized that CRISPRs can also regulate self gene expression by utilizing spacers that target self genes. By analyzing CRISPRs from 330 organisms we found that one in every 250 spacers is self-targeting, and that such self-targeting occurs in 18% of all CRISPR-bearing organisms. However, complete lack of conservation across species, combined with abundance of degraded repeats near self-targeting spacers, suggests that self-targeting is a form of autoimmunity rather than a regulatory mechanism. We propose that accidental incorporation of self nucleic acids by CRISPR can incur an autoimmune fitness cost, and this could explain the abundance of degraded CRISPR systems across prokaryotes.